Sweet desserts drive the search for disease-carrying mosquitoes

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ZETA, (STRI.SI.EDU).- As researchers ask which disease-carrying mosquito species will rule Panama’s Azuero Penninsula (and perhaps the world), they discover culinary delights along the way.

Kelly Bennett sets out from Gamboa at 4:30am, crossing to the western side of the Panama Canal on the tall, cable-stayed Centennial bridge. The Smithsonian post-doctoral fellow is on a six-hour trek to the Azuero Peninsula to track two mosquito species capable of lethal viral diseases: malaria, yellow fever, dengue, Zika and Chikungunya.

At 7am she meets Smithsonian research associate Jose Loaiza, who is also a senior scientist at Panama’s government research institute, INDICASAT, and professor at the University of Panama, for breakfast at a tiny fonda in Penonome. His research assistant Jose Ricardo Rovira and master’s student Alejandro Almanza polish off plates of eggs, tortillas, carimañolas (yucca pastries stuffed with meat) and bistec picado (beef stew) as Kelly and Jose finalize plans for the day.

The so-called “Mosquito team” will make this trip down the Azuero penninsula every month for the next three years, hoping to better understand the ecology of two of the world’s most dangerous organisms: Aedes aegypti and Aedes albopictus. Aedes mosquitos carry a wide range of viral and parasitic diseases.

“More than 245 different mosquito species live in Panama,” says Kelly. The challenge for the team is to discover which Aedes mosquito species are at each site and whether they have the right environmental conditions to breed and to infect people.

Aedes aegypti, from sub-Saharan Africa, first colonized Europe from the early 18th to the late 20th century. Now one of the most widespread mosquito species in the world, Aedes aegypti is still restricted to the tropics because it can’t survive winter. But surfing on recent heatwaves, it has begun to move both north and south.

Like rats and cockroaches, Aedes aegypti’s success goes hand-in-hand with humans’ explosive population growth. It feeds mostly during the day in the shade and indoors and breeds in standing water in containers and trash around homes.

Researchers in Panama have kept track of Aedes aegypti for more than 100 years since the Cuban doctor, Carlos Finlay first suspected that it carried yellow fever, the disease that foiled the French attempt to build the Panama Canal, killing thousands of workers. Subsequent, draconian measures to eradicate yellow fever imposed by the U.S. military included fining owners of flower vases and baptismal fonts where immature larvae were found. They succeeded in eliminating yellow fever, but Aedes aegypti mosquitoes are back.

Now another species, the Asian Tiger mosquito, is actively expanding around the world. Aedes albopictus arrived in Panama only about 15 years ago, hitchhiking in shipments of new and used tires. Its population doubled in France in the last two years. It was reported in Jamaica for the first time only about a month ago.

Tiger mosquitos bite aggressively during the daytime and carry many of the same diseases carried by Aedes aegypti. The most worrisome difference between the two species is that Aedes albopictus can live where it is cold. It has the potential to pick up a virus in one part of the world and infect people on another continent.

Turning south off of the PanAmerican highway at Divisa, the team passes through La Arena and Chitre and on to Las Tablas, famous for its yearly Carnival blowout parades.

They stop to pick up Madeleine Ducasa, biology student at the University of Panama’s campus in Chitre, who waits with her father under the overhang of a roadside bus stop. Jose’s grant from Panama’s secretariat for science, technology and innovation (SENACYT) requires that he works with university students at all levels: “I was really lucky to find an undergraduate so passionate about the subject at a university on the Azuero.”

The forty-some miles (68.7 kilometers) from Las Tablas to Tonosí takes nearly an hour-and-a-half—winding up hills clad in brown, sun-baked pastures bordered by leafy, living fences. Dogs sleep in the road as if dead, sprinting to safety split seconds before becoming roadkill. At the very top of the ridge, four old men slap dominoes down on a plastic table in an open fonda overlooking the Pacific. Then the road winds down to the coast.

Tonosí purportedly takes its name from the indigenous big-man, Tocona, whose people succumbed to smallpox and were eventually replaced by outlaws attempting to distance themselves from the Spanish crown. These days about 2300 people call the town home. A frontier town, it’s a gateway for tourists hoping to see sea turtles nesting on the beaches at Caña Island and adventurers buying last-minute supplies for a trek into Cerro Hoya National Park.

“We love to work on the Azuero,” he adds. “People out here invite us in for coffee. In some other parts of the country, it’s not like that. Last week we set out mosquito traps at 15 houses. We ask people if we can put these small black buckets in their gardens. We try to spread out the traps so that we sample the whole area. To avoid sampling eggs from the same female, we pick houses that are about 100 yards (91 meters) apart.”

Jose parks the pickup in front of the first house. A woman gets up from the hammock on the porch to greet them. Jose and Kelly show her the larvae floating in the trap recovered from her yard. “The mosquitos lay their eggs on the popsicle stick we place in the bucket. They hatch. The smallest larval stage comes out. Then the larvae pass through four more stages, getting bigger each time. And eventually they become adults and fly away. These little larvae are Aedes,” says Jose.

Madeleine pours the contents of the bucket into a plastic bag labeled with the location and date. Kelly wipes the bucket with a white cotton swab, dropping it into the bag with the stick. It’s covered with pinprick-sized mosquito eggs. “We won’t know if they’re Aedes aegypti or Aedes albopictus until we take them back to the lab and they emerge as adults,” she explains.

Alejandro puts the bags in a plus-sized red cooler in the back of the truck. If the water gets too warm, the larvae die.” When he’s not helping with the mosquito project, he’s working on his master’s thesis, a study of a nocturnal biting fly species called Lutzomyia that transmits the disfiguring disease, leishmaniasis.

At the next house, they smell lentils cooking. The water from the bucket is full of squirming larvae.

“See that big larva there? That’s a predatory mosquito called Toxorhynchites. We have to get it out or it will eat all of the others.” Kelly siphons it off with her turkey baster and transfers it to a smaller bag.

Everyone troops back to the truck. “We try to pick the houses without fences in case we have to go back when no-one’s at home.” Kelly says she’s learned to deal with passive-aggressive dogs as she goes from house to house: “always face the dog. It’s when you turn and run that they bite.”

They walk around the corner of the next house, greeted by the broad smile of a woman vigorously grating coconut into an aluminum cooking pot. Her husband has been lining up cement blocks to enlarge their back patio, but he takes a break at her bidding, going inside to get a paper cup filled with miel de caña, molasses. She plans to cook the grated coconut with this syrup, adding some vanilla, to make cocadas.

“I’m getting tired of grating,” the woman says. “You can help me out by eating some of this coconut.” Madeleine holds the cup as everyone dips uneven pieces of coco into the golden-brown syrup, a welcome mid-morning snack.

The woman on the next porch worries because rain continues to pour down as the researchers troop into her back yard in search of the bucket. “It’s fine,” says Jose, “We’re biólogos. We’re resistant.”

The next house is surrounded by an edible garden of plantains, bananas, yucca and pumpkin vines with bright orange flowers. As we take a look at a plant we don’t recognize, the owner emerges from the back balcony to explain that it’s a home remedy for cleaning the blood. When we comment on the abundance of cherry tomatoes bunching on the vine along the side of the house, he goes back inside and emerges again with a pale-yellow plastic bag mounded with very cold, sweet bite-sized tomatoes, nothing like the hard, white-pulped tomatoes at the super market.

Kelly walks past a rose bush in a sawed-off yellow plastic cooking-oil container to sample standing water in another flower pot with her turkey baster—no larvae there.

The water in the mosquito trap looks suspiciously clear.

While the students empty the bucket into a bag, Senior Rovira, an experienced entomologist at INDICASAT wearing a Smithsonian polo shirt, walks around the property looking for trash and containers of standing water. A half-melted plastic bottle on the ground nearby contains hundreds of larvae, leading us to suspect that the landlady may have emptied out the trap, replacing the water because she was nervous that the team had something to do with the government’s health department, which still fines people who have standing water harboring mosquito larvae on their property.

Back in the truck, they continue down the road. “The next house had mangoes behind it, on this side of the street. No, not this one.”

“Is it ok if we take some mangos?” Jose asks the elderly couple on the porch after we empty the trap. “These are the best! They taste like pineapple!” Jose picks up a hard, green mango from the ground and sends it sailing up through the branches where it rips through several bunches of leaves, downing two dangling fruits, which bounce off in random directions, rolling among the cement paving stones of the garden.

Alejandro’s Google map stops working because there’s no internet signal on his phone in this part of town. As a backup he has photos of all the trap locations. The next house on the list is made from broad blue boards. The first blue house we come to has a pair of twin Chihuahuas with matching red collars on the porch. “I don’t remember those,” says Madeleine.

Then they see another blue house set back from the road, behind a cloud of woodsmoke from a smouldering fire in a big oil can. “That’s the one. I remember the lady.”

It’s 11:30 and everyone’s starting to get hungry, which leads to Jose to start telling a story: “We went to La Arena to a bakery on the way back home, and as we were ordering Alejandro said ‘Please give me 8 mamallenas.’ [Mamallenas are usually a very heavy bread pudding, full of cinnamon and raisins.] And I said ‘What? Eight is not the number you want. That’s too many.’ And Rovira said ‘Please, give me two mamallenas!’ What?! I said ‘Hold on you guys.’

https://stri.si.edu/story/mosquitoes-and-mamallenas

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And then Alejandro said ‘Try a mamallena.’ So I bought one. And I bought a chicha de maracuya—passion fruit juice.”

“The mamallena was GREAT! I bought three more. I couldn’t resist and I ate all three on the way back to Panama. And I remembered that Yamibel wanted one, so Rovira gave me one to give her. She called me later and said ‘I have NEVER had such a good mamallena!’ The next time we came out here, she gave us money to bring back 12 mamallenas.”

And so now, back at my office at INDICASAT, everyone asks me if we’re bringing mamallenas with us when we come back from our trips to the Azuero.

And I say ‘No…we’re bringing mosquitos!’”

The team heads to a restaurant for a late lunch. This afternoon they will move on to Cacao, a more rural community. On Saturday, they’ll work their way back up the peninsula.

“Pretty much every house we visited today had mosquitoes in the traps,” Jose sums up. Albopictus is a very aggressive newcomer. We think albopictus may be pushing aegypti out in rural areas. Aegypti likes urban places. Albopictus likes rural places. That’s mostly because Aegypti rests inside houses and Albopictus rests on the vegetation outside.”

“Yes, so what we really want to know is ‘who wins and who loses when these species meet?’,” Kelly continues. She has been analyzing the data they already collected in 2016 and 2017.

“What if you calculate the distance from a major highway?” Jose suggests.

Jose explains that these mosquitoes basically live with us all of the time, almost like domesticated animals. “The density of mosquitoes here is really high. People may not have a cat or a dog, but they have Aedes, and they probably don’t even realize it. In really rural areas Aedes albopictus has an advantage, whereas in the city we find almost all Aedes aegypti. It’s in little towns like Tonosí where this drama is playing out, where diseases either take hold or they don’t. The mosquitos are here. It’s in these settings where it is decided whether an epidemic gets started or not.”

Make your own mamallena, with or without gluten and sugar
Crumble day-old bread to make three cups. Add a cup-and-a-half of whole milk and/or evaporated milk. Add half a stick of margarine, three or four eggs, vanilla extract, at least a teaspoon of cinnamon, a big handful of raisins and a generous pinch of salt. Beat until the bread dissolves. Sprinkle a buttered cake pan with about half a cup of sugar and more raisins and then pour in the batter. Bake at medium heat until, when you stick a knife in, it comes out clean and the mamallena is golden brown. For people like Kelly who are gluten intolerant, you can make a gluten-free version by substituting 3 cups of mashed ripe plantains for the bread. You can leave the sugar out of this version, too.

https://stri.si.edu/story/mosquitoes-and-mamallenas

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